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关键词： circulating tumor cells   CellSearch (R)   EpCAM   leukocytes   ACCEPT   deep Learning   classification   image analysis   non-small cell lung cancer  

摘要： In the CellSearch (R) system, blood is immunomagnetically enriched for epithelial cell adhesion molecule (EpCAM) expression and cells are stained with the nucleic acid dye 4'6-diamidino-2-phenylindole (DAPI), Cytokeratin-PE (CK), and CD45-APC. Only DAPI+/CK+ objects are presented to the operator to identify circulating tumor cells (CTC) and the identity of all other cells and potential undetected CTC remains unrevealed. Here, we used the open source imaging program Automatic CTC Classification, Enumeration and PhenoTyping (ACCEPT) to analyze all DAPI+ nuclei in EpCAM-enriched blood samples obtained from 192 metastatic non-small cell lung cancer (NSCLC) patients and 162 controls. Significantly larger numbers of nuclei were detected in 300 patient samples with an average and standard deviation of 73,570 +/- 74,948, as compared to 359 control samples with an average and standard deviation of 4191 +/- 4463 (p < 0.001). In patients, only 18% +/- 21% and in controls 23% 15% of the nuclei were identified as leukocytes or CTC. Adding CD16-PerCP for granulocyte staining, the use of an LED as the light source for CD45-APC excitation and plasma membrane staining obtained with wheat germ agglutinin significantly improved the classification of EpCAM-enriched cells, resulting in the identification of 94% +/- 5% of the cells. However, especially in patients, the origin of the unidentified cells remains unknown. Further studies are needed to determine if undetected EpCAM+/DAPI+/CK-/CD45-CTC is present among these cells.

关键词： Green coffee   Caffeine   Chlorogenic acid (5-caffeoylquinic acid)   HPLC-DAD   Multivariate curve resolution (MCR)   Classification   Partial least squares discriminant analysis (PLS-DA)   Soft independent modeling of class analogies (SIMCA)  

摘要： A chromatographic procedure (HPLC-DAD) using a relatively rapid gradient has been combined with a chemometric curve deconvolution method, multivariate curve resolution-alternating least squares (MCR-ALS), in order to quantify caffeine and chlorogenic acid in green coffee beans. Despite that the HPLC analysis (at these specific operating conditions) presents some coeluting peaks, MCR-ALS allowed their resolution and, consequently, the creation of a calibration curve to be used for the quantification of the analytes of interest;this procedure led to a high accuracy in the quantification of caffeine and chlorogenic acid present in the samples. In a second part of this study, the possibility of classifying the green coffee beans on the basis of their cultivar (Arabica or Robusta), by partial least squares discriminant analysis (PLS-DA) and soft independent modeling of class analogies (SIMCA), has been explored. SIMCA resulted in 100% of sensitivity and specificity for the Arabica class, while for the Robusta, it reached 66.7% of sensitivity and 100% of specificity, or 100% of sensitivity and 100% of specificity, depending on the extraction procedure followed prior to the chromatographic analysis;PLS-DA achieved 100% of correct classification independently of the procedure used for the extraction.

关键词： Pharmaceutical advertising   pharmaceutical products   drugs for anxiety   drugs for psychosis   drugs for anxiety   drugs for insomnia   sedatives  

摘要： Background: Many modern pharmaceutical products were launched during 1950-1980, as reflected in advertisements in the British Medical Journal (BMJ). One of the first therapeutic areas to benefit from novel effective medications was psychiatry. Methods: We examined BMJ advertising material between 1950 and 1980, including every other issue over six-month periods (October-March) in 1950/1951, 1955/1956, 1957/1958, 1960/1961, 1962/1963, 1965/1966, 1967/1968, 1970/1971, 1972/1973, 1975/1976, 1980/1981. We recorded numbers of adverts for all pharmaceutical products and for psychiatric drugs;we also recorded trade names, generic names and marketing company. Results: Advertising in BMJ peaked in the 1960s and declined markedly in the 1970s. Adverts for psychiatric drugs as a percentage of total pharmaceutical product advertising was broadly similar during 1955-1980, but with peaks in 1960/1961, 1970/1971 and 1975/1976, reflecting the entry of several novel compounds into the market. The peak marketing of antipsychotic drugs, sedatives and anxiolytic drugs was in 1960 and of antidepressants 1970. The time course of the rise of tricyclics and the switch from barbiturates to benzodiazepines can be seen. Drugs for psychiatry rose from ninth (1955/1956) to fourth (1975/1976) in terms of the number of products in the top 10 therapeutic areas. There is no evidence that they were advertised more aggressively (number of adverts/number of products). Conclusions: The birth of modern psychopharmacology is reflected in the advertising of psychiatric drugs in BMJ. Many drugs currently used, or their closely related successors, were launched in the early to mid-1960s. This rise in modern pharmaceuticals preceded several other major therapeutic areas.

关键词： Text classification   Semi-supervised learning   Sample extension   Wikipedia  

摘要： Text classification is a topic in natural language processing that is particularly useful for Internet information processing. Methods based on supervised learning require a large amount of manually annotated training samples. The annotation of training samples is time consuming, and performance relies heavily on the quality of the training samples. This paper presents a text classification method based on sample extension. The extension is based on the correlation of the labeled sample data and the concepts in Wikipedia. Combined with the rich link relationships between concepts, we selected appropriate articles from Wikipedia to expand the training sample set. By introducing the large amount of rich semantic concept pages that are contained in Wikipedia along with links that are related to different pages, our approach enhances the performance and generalization of the classifier. Experiments demonstrate that the performance of the method proposed in this paper is better than that of both supervised and semi-supervised methods.

关键词： 食品检验检测实验   室质量量度  

摘要： 本文重点分析了食品检验检测实验室中质量量度的作用,为以后我国食品质量管理模式发展提出新的思维途径。

关键词： 妇科炎症   感染   念珠菌   检验方法  

摘要： 目的探讨妇科炎症感染中念珠菌检验方法的临床应用价值。方法选2016年10月至2017年10月我院所收治的90例念珠菌阴道感染的妇科炎症患者作为观察对象,将90例患者分为A、B、C三组,A组采用镜检方法进行检验、B组采用凝集法进行检查、C组采用培养法进行检验,观察三组检验方法的临床效果。结果 A组检出阳性的患者有24例,B组检出阳性的患者有26例,C组检出阳性的患者有25例。三组检验方法进行比较,各数据差异并无统计意义(P> 0.05)。结论在妇科炎症感染中念珠菌检验的方法主要是镜检法、凝集法、培养法等方式,三种方法的阳性检测率相对较高,在临床疾病诊断当中具备重要的参考价值,在临床上具有重要意义。

关键词： 医学检验   护理   标本采集  

摘要： 目的观察分析精细化护理对医学检验质量的影响效果。方法本研究选择我院肾内科精细化护理实施前(2017年10月-12月)和精细化护理实施后(2018年1月-3月)递交至检验科的标本各100份为研究样本,实施前递交标本为普通护理标本采集组,实施后递交标本为精细化护理标本采集组,对上述两组标本的质量合格率加以比较研究。结果精细化护理标本采集组递交标本的质量合格率显著高于普通护理标本采集组(P<0.05)。结论加强临床护理人员标本采集运送知识培训,将精细化护理理念融入于标本采集工作之中,可显著提升标本质量合格率。

关键词： 卫生理化检验   疾病预防控制   作用   分析  

摘要： 当前,随着我国经济与社会的蓬勃发展,一些商家受到利益的驱使,在食品生产中大量使用化学添加剂或者有害物品,对公众的身体健康带来了严重的威胁,食品安全事件不断涌现,对我国社会的稳定带来一定程度的负面影响,卫生安全逐渐受到公众和社会的高度重视,理化检验作为控制疾病、食品检测的重要手段,其在预防以及检验食品安全中起到关键作用,同时也在预防和控制疾病中发挥了积极作用。因此,我国相关部门需要给予理化检验工作以高度重视,在实际工作中解放思想、更新设备、提升技术,切实发挥理化检验的功能与效应,为我国经济发展和社会稳定夯实基础。在此背景下,文章主要针对卫生理化检验在疾病预防控制中的作用进行分析和阐述,希望给予我国相关行业以参考和借鉴。

关键词： 痕迹检验技术   刑事侦查   作用  

摘要： 痕迹检验技术是公安部门在推进侦查工作中比较常用的一种技术手段,因为后期所有的侦查工作都要借助痕迹检验的成果,可以说是整个侦查工作中的基础性工作。除了为侦查工作提供信息和数据外,痕迹检验技术还能提高侦查工作的效率,推进侦查工作的进程。但是在我国的刑事侦查工作中,对痕迹检验技术的运用还存在很多误区,这就给侦查工作的推进造成了很大的阻碍,因此强化对痕迹检验技术的认识是非常有必要的。本文结合痕迹检验技术在刑事侦查工作的应用,对其具体作用提出了自己的看法,仅供参考。

关键词： 生化检验   病因病机   肝硬化   臌胀  

摘要： 目的探讨生化检验项目运用在肝硬化疾病诊断中的临床效果及其中医病因。方法选取2015年9月—2016年12月收治的56例肝硬化患者为观察组,同期健康体检者60例为对照组,行生化检验,对比2组检测结果。结果观察组TP CHE和ALB水平明显低于对照组,ALT、γ-GT、TBIL、AST、DBIL、ACP水平明显高于对照组,差异有统计学意义(P<0.05)。结论中医学认为肝硬化疾病的发生与肝肾阴虚、脾肾阳虚、瘀血阻滞、湿热蕴结以及气滞湿阻等有关,及时行生化检验可以提高诊断准确率。