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关键词： Pararhizobium haloflavum   Soil samples   Sewage treatment tank   Taxonomy  

摘要： A Gram-stain negative, aerobic, motile and ovoid- to rod-shaped bacteria strain, designated XC0140(T), was isolated from soil samples near the sewage treatment tank of a chemical factory in Zhejiang Province, China, and subjected to polyphasic taxonomic investigation. Strain XC0140(T) grew at 10-37 A degrees C and pH 6.0-9.0 (optimum, 35 A degrees C and pH 7.5) and with 0-17% (w/v) NaCl (optimum, 1%). According to phylogenetic analysis based on 16S rRNA gene sequences, strain XC0140(T) was assigned to the genus Pararhizobium with high 16S rRNA gene sequence similarity of 95.97% to "Pararhizobium helanshanense CCNWQTX14(T"), followed by Pararhizobium sphaerophysae CCNWGS0238(T) (95.95%). Chemotaxonomic analysis showed that strain XC0140(T) contains ubiquinone-10 as the predominant respiratory quinone and possessed summed feature 8 (comprising C-18: 1 omega 7c and/or omega 6c), 11-methyl C-18:1 omega 7c, C-18: 0 and C-16: 0 as predominant forms of fatty acids. The polar lipids of strain XC0140(T) consisted of seven phospholipids (PL), two aminolipids (AL), one glycolipid (GL) and three unidentified lipids (L1, L2 and L3). The DNA G+C content was 62.7 mol%. Based on the polyphasic taxonomic characterization, strain XC0140(T) is considered to represent a novel species of the genus Pararhizobium, for which the name Pararhizobium haloflavum sp. nov. is proposed. (type strain XC0140(T) = MCCC 1K03228(T) = KCTC 52582(T)).

关键词： cancer   cervix   Raman spectroscopy   screening  

摘要： The ability to provide quantitative, objective and automated pathological analysis would provide enormous benefits for national cancer screening programmes, in terms of both resource reduction and improved patient wellbeing. The move towards molecular pathology through spectroscopic methods shows great promise, but has been restricted by spectral quality, acquisition times and lack of direct clinical application. In this paper, we present the application of wavelength modulated Raman spectroscopy for the automated label- and fluorescence-free classification of fixed squamous epithelial cells in suspension, such as those produced during a cervical smear test. Direct comparison with standard Raman spectroscopy shows marked improvement of sensitivity and specificity when considering both human papillomavirus (sensitivity +12.0%, specificity +5.3%) and transformation status (sensitivity +10.3%, specificity +11.1%). Studies on the impact of intracellular sampling location and storage effects suggest that wavelength modulated Raman spectroscopy is sufficiently robust to be used in fixed cell classification, but requires further investigations of potential sources of molecular variation in order to improve current clinical tools.

关键词： Games   Instructional quality   Mathematical interactions   Mathematics   Student thinking  

摘要： This study explored the design and implementation of the Cover-up and Un-cover games, two manipulative-based fraction games, in 14 fifth-grade classrooms. We examined how the fraction concepts were integrated into the game design and explored the nature of teacher-student interactions during games using lesson videos. Our examination showed that interactions focused on game progress, rules, and turn taking with little exploration of strategies or the underlying mathematics. To compare the quality of teacher-student interactions during games with interaction quality during other instructional activities, we coded 5 videotaped lessons from each of the 14 classrooms. Statistical results from a dependent t test indicated that teacher-student interactions were of statistically significant lower quality during games than during non-game segments with similar instructional purpose. Teachers might benefit from additional curriculum support and training to implement games as rich mathematical learning opportunities.

关键词： 检验机构   特种设备   安全管理   作用  

摘要： 在当今中国经济飞速发展的时代背景下,科学发展速度惊人,科技不断更新改进,各种新型的特种设备被研发、制造并安装,应用在各行各业中。而很多特种设备都具有高危险性,一旦发生安全事故就会造成严重后果,带来不可控的伤害。因此,特种设备检验机构的检验检测工作具有举足轻重的意义。为防止和减少事故的发生、保障人民群众的安全,为特种设备创造安全运行的条件,从而增加使用单位的企业经济效益。本文重点对特种设备检验机构在特种设备安全管理方面起到的作用进行了细致的分析。

关键词： 血涂片分析   血常规检验   血细胞分析仪  

摘要： 目的:探讨血涂片分析在血常规检验中的作用。方法:选取84例进行血常规检验的受检者为研究对象,所有受检者均接受血涂片和血细胞分析仪检测,分析检测结果。结果:84例受检者经血涂片检查发现正常标本56例,异常标本28例,血细胞分析仪检测正常标本44例,异常标本40例,两种检验方法异常标本检出率差异有统计学意义(P<0.05)。血涂片检查中发现有1例标本中出现不同程度空泡、2例样本中有少许中毒颗粒、3例出现中心染色区扩大问题。结论:在血常规检查中应用血涂片能提高检查的准确率,减少误诊与漏诊,便于医者准确判断患者身体状况,具有较高应用价值。

关键词： 河南省人民政府办公厅   出入境检验检疫部门   经济发展   职能作用   开放型   海关   省直管县   全面开放  

摘要： 各省辖市、省直管县（市）人民政府，省人民政府各部门：为进一步发挥海关和出入境检验检疫部门职能作用，促进我省开放型经济发展，打造内陆开放高地，加快形成全面开放新格局。经省政府同意，现提出如下意见。

关键词： 内部督查机制   检验检疫   质量管理体系  

摘要： 本文结合内部督查工作的体会,从工作质量内部督查机制的定义、内容和流程及改进建议等方面对内部督查机制在体系中的作用进行阐述,为检验检疫内部督查机制的完善,提高质量管理体系的有效性提供参考。

关键词： 社会适应双功能模型   师生关系   友谊质量  

摘要： 采用全国七城市大型调查数据考察社会适应双功能模型在中学生群体中的适用性及其校园人际关系的预测作用。结果发现,(1)根据全国城市中学生常模数据划分为四种适应类型:适应型(74.1%)、脆弱型(9.9%)、矛盾型(8.8%)和高危型(7.1%);(2)不同适应类型的校园人际关系存在显著差异:在师生关系和同伴友谊的积极指标上,呈现出适应型>矛盾型>脆弱型>高危型,在关系冲突指标上则为高危型>矛盾型>脆弱型>适应型。(3)多项Logistic回归分析表明,校园人际关系不同指标能显著预测不同的适应类型。社会适应双功能模型适用于城市中学生群体,校园人际关系(尤其是同伴友谊)是预测不同适应类型的敏感指标。

关键词： 溶血   临床生化检验   影响作用   防控举措  

摘要： 目的观察及分析溶血对临床生化检验质量产生的影响情况。方法本研究对象是130例于我院实施健康体检的人员。对体检者采集血液并在两支试管中分配,其中一管实施常规处理送检,另外一管实施人工溶血送检。对于临床生化检验指标结果进行统计和观察。结果通过对比丙氨酸转移酶、乳酸脱氢酶、血钾及总胆固醇含量几项指标,结果显示溶血组显著高于常规组(P<0.05);对比两组的三酰甘油、尿素氮、血糖、血尿酸及肌酐等指标,组间未见明显的差异性(P<0.05)。结论临床生化检验期间积极防控溶血现象尤为关键,溶血问题会严重影响到检测质量。

关键词： 卡式微柱凝胶技术   聚凝胺法   不规则抗体检验   输血反应  

摘要： 目的探讨卡式微柱凝胶技术在ABO和RhD血型鉴定及输血前红细胞不规则抗体检验中的作用。方法采用卡式微柱凝胶技术和聚凝胺法鉴定西安交通大学医学院附属三二〇一医院1 175例输血患者ABO和RhD血型及红细胞不规则抗体,观察两种方法检测ABO和RhD血型及不规则抗体检出情况,并记录科室分布情况。结果血型鉴定结果显示,卡式微柱凝胶技术鉴定ABO血型的一次性准确率为99.83%,显著高于聚凝胺法的98.72%(P=0.002);卡式微柱凝胶技术鉴定RhD血型的一次性准确率为99.91%,显著高于聚凝胺法的99.32%(P=0.039)。卡式微柱凝胶技术不规则抗体检测中16例患者表现为阳性,阳性率为1.36%;聚凝胺法不规则抗体检测中10例患者表现为阳性,阳性率0.85%。26例不规则抗体阳性患者中血液科、肾病科和感染科共21例(84.00%),其他科室5例(19.23%);有输血史者22例(88.00%),有妊娠史者12例(48.00%)。结论卡式微柱凝胶技术在ABO和RhD血型鉴定及输血前红细胞不规则抗体检验中结果容易判断且稳定、可靠,操作过程简单易行,能有效保证输血的安全性。