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关键词： 生化检验   血清   终点法   改良终点法  

摘要： 目的分析改良终点法与原终点法在临床生化检验中的区别和各自的应用优势,以优化临床生化检验技术。方法利用改良终点法与原终点法进行生化检验,收集200份血清标本,利用全自动生化分析仪连续测量10 d,对两种方法之间的线性浓度以及相关性进行分析。结果在相关性结果方面,改良终点法与原终点法的差异不显著(P>0.05)。在正常浓度情况下,改良终点法的总CV以及批内CV均显著高于原终点法(P<0.05);在异常浓度情况下,改良终点法的总CV以及批内CV值均显著低于原终点法(P<0.05),经线性分析,中、低浓度下,改良终点法和原终点法之间存在良好的线性关系。高浓度条件下,改良终点法的线性浓度显著优于原终点法(P<0.05)。结论对高浓度标本进行生化检验的过程中,应用改良终点法可以获得较原终点法更好的效果,精密度更高,测定的线性范围更广。

关键词： 微生物检验   感染性疾病   预防   诊断  

摘要： 临床医学通过微生物形态学对病原体进行判断.根据感染性疾病病原体的不同,针对性地选择治疗方法,从而为感染性疾病的治疗提供治疗依据.本研究简要分析了微生物检验在感染性疾病及相关病患的预防和诊断治疗中的作用,希望能为临床医学工作者提供一定的参考.

关键词： METASTASIS   GENE expression   CANCER invasiveness   EPIGENOMICS   DNA methylation   BIOCHEMICAL markers   BREAST cancer  

摘要： Motivation: Cancer development and progression is driven by a complex pattern of genomic and epigenomic perturbations. Both types of perturbations can affect gene expression levels and disease outcome. Integrative analysis of cancer genomics data may therefore improve detection of perturbed genes and prediction of disease state. As different data types are usually dependent, analysis based on independence assumptions will make inefficient use of the data and potentially lead to false conclusions. Model: Here, we present PINCAGE (Probabilistic INtegration of CAncer GEnomics data), a method that uses probabilistic integration of cancer genomics data for combined evaluation of RNA-seq gene expression and 450k array DNA methylation measurements of promoters as well as gene bodies. It models the dependence between expression and methylation using modular graphical models, which also allows future inclusion of additional data types. Results: We apply our approach to a Breast Invasive Carcinoma dataset from The Cancer Genome Atlas consortium, which includes 82 adjacent normal and 730 cancer samples. We identify new biomarker candidates of breast cancer development (PTF1A, RABIF, RAG1AP1, TIMM17A, LOC148145) and progression (SERPINE3, ZNF706). PINCAGE discriminates better between normal and tumour tissue and between progressing and non-progressing tumours in comparison with established methods that assume independence between tested data types, especially when using evidence from multiple genes. Our method can be applied to any type of cancer or, more generally, to any genomic disease for which sufficient amount of molecular data is available.

关键词： DNA检验技术   交通肇事   事故案件   鉴定技术   案件处理  

摘要： DNA检验技术被誉为“新一代的证据之王”，在交通事故案件的处理中得到了十分广泛的应用。DNA检验技术的误差范围在亿分之一以下，提高了法医物证鉴定技术的准确性，使之成为了交通肇事案件处理中的重要工具。

关键词： Age estimation   Skeletal age   Dental age   Forensic odontology   Age assessment   Linear Discriminant Analysis  

摘要： Background: Dental and skeletal maturation have proved to be reliable evidence for estimating age of children and prior studies and internationally accredited guidelines recommend to evaluate both evidence in the same subject to reduce error in age prediction. Nevertheless the ethical and legal justification of procedures that imply a double exposition of children stands as a relevant issue. This study aims to evaluate the accuracy of age estimation provided by a combination of skeletal and dental methods applied in the same sample of children. Materials and methods: The sample consisted of 274 orthopantomographies and left hand-wrist X-rays of Italian children, (aged between 6 and 17 years) taken on the same day. Greulich and Pyle's (GP), Tanner-Whitehouse's version 3 (TW3) and Willems' (W) and the Demirjian's (D) methods were respectively applied for estimating skeletal and dental age. A combination of skeletal and dental age estimates through Linear Discriminant Analysis (LDA) is proposed to obtain a classifier respect to an age threshold. Results: The combination of D and TW3 obtained an improvement of accuracy in classifying female subjects respect to the 12 years threshold respect to the original methods (from about 77% using either original methods to 83.3% combining TW3 + D) as well as a consistent reduction of false positives rate (from 17% to 21% for original methods to 5.6% with TW3 + D). For males the LDA classifier (based on TW3 and W) enable a small improvement in accuracy, whilst the decreasing of false positives was as noticeable as for females (from 17.6 to 14.1% for original methods to 6.2% combining TW3 + W). Conclusions: Although the study is influenced by the limited size and the uneven age distribution of the sample, the present findings support the conclusion that age assessment procedures based on both dental and skeletal age estimation can improve the accuracy and reduce the occurrence of false positives. (C) 2016 Elsevier Ireland Ltd. All r

关键词： 工具痕迹   工具痕迹检验技术   刑事侦查  

摘要： 痕迹检验是指侦查人员运用痕迹学,化验学等理论,对案发现场遗留的各种痕迹进行搜集比对,并推测物证痕迹与犯罪事实之间在犯罪现场中的联系,而工具痕迹是最为多见的一种犯罪痕迹。在判断犯罪实施手段、方法中,工具痕迹起到了很大的作用,它不仅可以判断犯罪工具种类,甚至还可以判断犯罪行为人的职业等特点。工具痕迹是刑事痕迹检验技术工作的一个重要分支,也是刑事犯罪现场出现率较高的痕迹物证。

关键词： 产品质量检验   质量技术监督   措施  

摘要： 由于人们生活条件越来越好,更加注重对于物质质量方面的要求,所以,在进行技术监督工作中,应该充分认识到产品质量检测的重要内容。对产品质量检验在质量技术监督中的作用进行了探讨。

关键词： Consolidation & merger of corporations  

摘要： The article reports that the drug manufacturing firm Recipharm has agreed to acquire the contract pharmaceutical business of Kemwell, including facilities in Sweden, India, and the U.S., that highlights the merging of chemical and finished drug manufacturing.

关键词： 硬度检测   金属检验   作用  

摘要： 随着经济以及社会的不断发展,工业化生产以及人们对于生活的基本需求不断的提升,对于基础性材料的要求也是随着时代的发展,逐渐的升高,其中金属材料的应用取得了前所未有的进展。在金属的基本检验工作中,硬度检测具有很重要的地位,通过对金属材料进行合理的硬度检测等技术,对材料中可能出现的部件或者材料出现不能正常的工作的现象进行合理的完善和改进,本文就上述的基本问题和技术进行了一定的探析,同时对分析的基本结果进行合理的分析和描述,对基本的检测手段以及对部件的基本处理方式进行比较合理的阐述,进而就能够对所进行监测的部件进行充分的判断,确定其是否对现场的基本应用情况有一定的发展前景。

关键词： 微生物检验   医院感染   控制作用  

摘要： 探讨微生物检验在医院感染控制中的积极作用。方法:随机抽取我市市直医院200例2014年1月到2015年10月间收治的200例患者进行临床分组研究,对照组和观察组各100人,对照组采用常规方法进行临床治疗和护理,观察组则在此基础之上重点进行微生物检验,然后对比两者在感染方面的差异性。结果:从感染的轻度、中度和重度三个方面来看,对照组患者比例为11%、5%和3%,而观察组则分别为4%、2%和1%,这三者之间的差异性检验发现并不存在着显著差异,但是从感染总数上来看,对照组患者的感染比例为19%,观察组则为7%,统计分析结果在0.05水平上显著,也就是说,采用微生物检验的观察组感染比例明显低于未采用微生物检验的对照组。结论:在医院感染控制中加强对于微生物检验的应用是极为重要的一环,值得在今后的医院临床治疗中进行推广应用。