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关键词： PROTEIN analysis   PROTEIN metabolism   GENES   MICROSCOPY   METHODOLOGY   BIOCHEMISTRY   BIOLOGICAL assay   CELL receptors   CELLULAR signal transduction   PHENOMENOLOGY   FINANCING of research   YEAST  

摘要： Interaction between P-factor, a peptide pheromone composed of 23 amino acid residues, and its pheromone receptor, Mam2, on the cell surface of the fission yeast Schizosaccharomyces pombe was examined by an atomic force microscope (AFM). An AFM tip was modified with P-factor derivatives to perform force curve measurements. The specific interaction force between P-factor and Mam2 was calculated to be around 120 pN at a probe speed of 1.74 mu m/s. When the AFM tip was modified with truncated P-factor derivative lacking C-terminal Leu, the specific interaction between the tip and the cell surface was not observed. These results were also confirmed with an assay system using a green fluorescent protein (GFP) reporter gene to monitor the activation level of signal transduction following the interaction of Mam2 with P-factor.

关键词： 定金   《合同法》   当事人   房屋买卖   违约方  

摘要： 案例2011年8月,张某与王某签订了一份《房屋买卖协议》,约定王某将自建的120平方米房屋卖给张某,价格为20万元。张某先行给付4万元定金,余款16万元于王某交房时交清,如一方违约,则应承担相应法律责任。合同签订后,张某给付了王某4万元定金,王某给张某出具了收据。事后不久,恰逢房产部门到王某房屋所在区域做房屋动迁摸底调查。王某为获得高额拆迁款,遂以被张某欺骗为由,多次找张某要求退还定金,不再卖房,但遭张某拒绝。协商未果,王某不再理会张某,将房屋以更高价格出售给李某,并办理了过户,张某得知后起诉至法院。疑惑1.王某不履行协议的行为是否应予支持?2.协议中的4万元定金该如何处理?

关键词： 木材检验   木材缴库   拨付   作用  

摘要： 木材检验工作在森工企业的生产环节中,占有非常重要的地位,特别是在贮木场及山上林场的木材接验收及缴库拨付中,发挥的作用更为明显,森工企业要想实现其经济效益的最大化,就必须做好木材的检验工作。现阶段我国木材缴库和拨付中还存在很多问题,本文为着重从木材检测的角度来进行相关分析,并提出了一些具体的可行措施,以期实现森工企业生产管理水平的有效提高。

关键词： 木材检验工作   天然林保护工程   资源   经济效益  

摘要： 天然林的形成年限时间长,近年来的各种违规操作行为已经给其带来了严重的破坏,基于现状,国家的林业管理部门和国内的群众都开始意识到天然林保护工程的重要性。实践表明木材检验工作是提高天然林保护能力的一个有效手段。文章将重点论述木材检验工作队天然林保护工程的影响和意义,以全面提高天然林保护工作的水平。

关键词： 合同结构   工程造价   总包   平行承包  

摘要： 不同的合同结构形式对工程造价有不同影响，选择哪种合同结构形式需考虑影响造价的主要因素有，业主自身的管理团队、总包单位的能力、减免税费、项目开办费等。

摘要： A pooling design can be used as a powerful strategy to compensate for limited amounts of samples or high biological variation. In this paper, we perform a comparative study to model and quantify the effects of virtual pooling on the performance of the widely applied classifiers, support vector machines (SVMs), randomforest (RF), k-nearest neighbors (k-NN), penalized logistic regression (PLR), and prediction analysis for microarrays (PAMs). We evaluate a variety of experimental designs using mock omics datasets with varying levels of pool sizes and considering effects from feature selection. Our results show that feature selection significantly improves classifier performance for non-pooled and pooled data. All investigated classifiers yield lower misclassification rates with smaller pool sizes. RF mainly outperforms other investigated algorithms, while accuracy levels are comparable among all the remaining ones. Guidelines are derived to identify an optimal pooling scheme for obtaining adequate predictive power and, hence, to motivate a study design that meets best experimental objectives and budgetary conditions, including time constraints.

关键词： Girls in science   Science identities  

摘要： At a 5-day summer camp designed for middle-school girls (N = 50), fifth through ninth-grade students were able to identify with individual scientists and learn more about the science field. Data from the girls' journals, pictorial representations, and field notes demonstrated that these young women related to scientists who actively engaged them in hands-on, problem-based activities and had a sense of humor while doing so. Girls closely identified with science fields that offer an opportunity for travel and for interaction with animals. In addition, it is evident that the girls' personal and social interactions shaped their perceptions of scientists and helped them internalize their experiences as members of a science community in relation to their overall science identities.

关键词： 合法形式掩盖非法目的   无效合同   撤销权  

摘要： 无效合同的确认是合同法理论和司法实践中的重要问题,尽管我国《合同法》对无效合同的确认标准做了原则性规定,但理论和实务上仍存在一些模糊的认识和做法.本文试探讨无效合同制度中以合法形式掩盖非法目的合同的认定应注意的几个问题,更加清晰地划清相关问题之间的界限.

关键词： 木材检验   重要作用  

摘要： 木材检验工作贯穿于木材生产经营的全过程,它从原条的合理量材设计开始一直到木材产品的最后售出,这些过程,与木材产值、产量、质量、品种以及出材率等主要经济技术指标息息相关.木材检验进行的好坏直接影响企业的经济效益和森林资源的合理利用.

关键词： 痕迹检验   刑事侦查  

摘要： 痕迹检验技术是公安机关侦查人员综合运用痕迹检验理论、方法检验犯罪现场遗留下的各项痕迹,以确定犯罪痕迹与犯罪嫌疑人实施犯罪行为之间的关系的刑事侦查方法,是刑事侦查技术的重要组成部分。痕迹检验技术主要集中于对犯罪现场遗留的手印、足印等痕迹进行提取、分析和比对,从而精确地锁定犯罪嫌疑人身份特征,提高刑事侦查工作效率。笔者通过具体分析痕迹检验技术的概念、主要特点以及其在刑事侦查工作中的作用,结合刑事案件侦查工作的实际提出了如何更为有效地在刑事侦查工作中运用痕迹检验技术。